Technology Projects > Marine BioTechnology > Activities

Activities


Marine Algal Biotechnology

  • For demonstrating large scale mass culture of marine microalgae for nutraceutical production in raceway ponds at seafront facility Pamanji, AP. The construction of raceway ponds have completed and installation of seawater intake is in progress.
  • Pressurized liquid extraction (PLE) was used to extract astaxanthin from Thalassiosira punctigera isolated from Antarctica using accelerated solvent extractor. Astaxanthin extraction in temperature (60-100 ᵒC) and pressure (50-150 bar) were tested and maximum astaxanthin yield 31.34 ±  1.36 mg/g was obtained at 80ᵒC, 70 bar and 20 minutes.
  • A two stage batch culture was tested with salinities 30 psu and 60 psu for astaxanthin accumulation in Dunaliella tertiolecta, Dunaliella salina, Dunaliella - Chlorella somatic hybrids (N150 and N200). The astaxanthin yield increased in D. tertiolecta from 6.14 ± 0.54 mg/L to 8.28 ± 0.41mg/L and the total carotenoids increased from 16.06 ± 0.66 mg/L to 26.22 ± 0.81 mg/L when the  salinity was increased from 30 psu to 60 psu.
  • Phylogentic analysis of six diatoms isolated from Antarctic Brokeness peninsula was completed using nuclear-encoded small sub unit (SSU) r DNA.
  • The macroalgae Acanthophores picifera and Gracilaria sp. collected from Andaman are being cultured in bamboo raft and the growth rate is being monitored. Liqid biofertilizer and biostimulant production from Sargassum cinerium and Hormophysa was carried out by anaerobic fermentation method.

Microbial  Biotechnology

  • New taxa of Streptomyces olivaceus NIOT-Ch-40 producing an active compound piericidin was isolated from sediment sampled collected from 2000 m depth.
  • The polyketide synthase-I, PKS-II and AHBA synthase, NRPS genes deep sea Streptomyces fenghuangensis NIOT.Ch.34 were amplified. The cloning and sequencing of NRPS and PKSII genes showed hits to ABC transporter substrate-binding protein and Streptomyces sp. JAJ-06 beta-ketoacyl-acyl carrier protein synthase.
  • Piezotolerance Ascotricha sp. isolated from 1235 m deep-sea sediment was successfully cultured in high pressure-low temperature (100 bar pressure and 20ºC) microbial cultivation system. Ascotricha produced 4 molecules  bioactive molecules such as diphenylmethane, 1,6-Cyclodecadiene, sesquiterpenes and dihydro-cis-alpha-copaene-8-ol.
  • Molecular screening, identification, characterization and in silico analysis of the trehalose biosynthesis genes in marine actinobacteria was performed with 78 nos. of Streptomyces sp. and 28 nos. of Nocardia sp.
  • Hydrocarbon degrading fungus that utilizes spent engine oil and crude oil as carbon and energy source was isolated from marine surface sediment collected at 3538 m depth off-mangalore. Self-immobilized fungus degraded  >85% of total hydrocarbons after 10 days of incubation with 1% (v/v) of spent engine oil at 28 ± 2ºC.
  • A total of 527 morphologically different representative bacterial colonies were isolated from the water sample collected from Arabian sea. Based on the biochemical characterization the bacterial isolates were categorized under 13 Genera, of which Bacillus sp., Oceanobacillus sp. and Shewanella sp. were the dominant genera.

Open Sea Cage Culture

  • Survey on availability of milkfish seed in Sangumal, Dhanuskodi, Kundhukkal, Mandapam and Vethalai was carried out and identify availability large number of milk fish seed during the month October to November.
  • As a part of brood stock development Caranx ignobilis  average body weight 3.5 and seabass, Lates calcarifer  of average body weigh 205 kg were stocked in 9 m wide open sea cages at North Bay, Port Blair. The growth performances of these brood stocks are being continuously monitored.
  • As part of collaborative programme between CIARI and ACOSTI in open sea cage culture nursery rearing of seabass seeds (1.5 –2 cm) obtained from CIBA, is being carried out at CIARI Marine Hill hatchery for stocking in the open sea cages.
  • Periodical cleaning maintenance for submersible cages has been carried out and long term performance of the prototype submersible cage is being evaluated.
  • The fishermen self help group engaged in open sea cage culture with the technical support of NIOT participated in World Brackishwater Aquaculture Conference and presented their success in open cage culture of seabass and cobia at Olaikuda in farmer’s conclave session. 

Establishment of Ballast Water Treatment Technologies – Test Facility

  • The physico-chemical parameters of the coastal water off Pamanji coast was continuously monitored and open sea was found to be suitable as a source of intake water with the addition of suitable surrogates as per the IMO G8 guideline.
  • Zooplankton survival/ viability were assessed using the standardized protocols of neutral red uptake and the method was successfully standardized (for live/dead screening) for its applicability in screening zooplankton viability.
  • Geotechnical investigation was completed and the specification for construction of trestle based seawater intake system has been completed. The process of invitation of open tender for construction of the ballast water tanks and trestle is in progress.

Collaborations
MoU between NIOT and CIBA was signed on 25th September 2018 in the presence of Secretary, MoES for the mutual cooperation of research activities in the areas of hatchery technology for fish seed production, feed processing technology & nutrition and health management of fishes in offshore cage culture programmes.
MoU between Andaman & Nicobar Administration and NIOT was signed on 20th January 2019 for Technological Initiatives